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. 2020 Jan 17;9(1):118. doi: 10.3390/plants9010118

Figure 2.

Figure 2

HPTLC chromatograms for the qualitative determination of flavan-3-ols and proanthocyanidins in STSs from leaves (2 μL, 50 mg/mL) of Japanese (track 6), Bohemian (track 7) and giant (track 8) knotweed based on standards of (−)-gallocatechin gallate (0.2 µg; track 1), (−)- catechin gallate (0.2 µg; track 2), procyanidin C1 (0.3 µg; track 3), procyanidin B3 (0.2 µg; track 4), (−)-epicatechin (0.1 µg; track 5, higher RF), (−)-epigallocatechin (0.2 µg; track 5, lower RF), (+)- catechin (0.1 µg; track 9, higher RF) (−)-gallocatechin (0.2 µg; track 9, lower RF), (−)-epicatechin gallate (0.2 µg; track 10), procyanidin B1 (0.2 µg; track 11), procyanidin B2 (0.2 µg; track 12) (−)- epigallocatechin gallate (0.2 µg; track 13). The HPTLC silica gel plate was developed with the developing solvent toluene-acetone-formic acid (3:6:1, v/v) and documented after derivatization with DMACA detection reagent at white light illumination.