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. 2020 Jan 16;9(1):114. doi: 10.3390/plants9010114

Figure 1.

Figure 1

Transcriptional activity and functional analysis of citrus FLOWERING LOCUS C homolog (PtFLC). (a) Structural comparison of five alternatively spliced PtFLC transcripts, rectangular boxes indicate exons and lines indicate introns. (b) Transcriptional activation analysis of five alternatively spliced PtFLC transcripts in yeast cells. Yeast cells carrying pBD-PtFLCV1, pBD-PtFLCV2/3, pBD-PtFLCV4, pBD-PtFLCV5, pGBKT7 empty vector (as a negative control), or the positive control (pCL) were streaked on SD/-Trp/-His medium supplemented with 5-bromo-4-chloro-3-indolyl-α-galactoside (X-α-Gal) and 3-amino-1,2,4-triazole (3-AT). (c) Phenotype analysis of PtFLC transgenic Arabidopsis. (d) Numbers of leaves and days to flowering of transgenic plants from five PtFLC transcripts at time of flowering stage under long day. Asterisks indicate significant differences (Student’s t-test): ** P < 0.01.