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. 2020 Jan 16;9(1):114. doi: 10.3390/plants9010114

Figure 3.

Figure 3

PtHB13 directly binds to the promoter of PtFLC. (a) Sequence analysis of PtHB13 protein with its homolog protein. PtHB13 from Citrus trifoliate, MDP0000423596 from Malus domestica, Potri.010G093400.1 from Populus trichocarpa, Ciclev10032279m from Citrus clementina, AT1G69780.1 from Arabidopsis, Zm00008a000472 T01 from Zea mays, and Os03g07450.1 from Oryza sativa. Heavy red line indicates conserved domain of these proteins. (b) Schematic diagrams of proPtFLC and constructs of yeast one-hybrid assay. proFLC indicates the promoter fragment containing a bZIP910 site, while proFLCm is mutated form of proFLC. (c) Schematic diagrams of effector and reporter vectors for dual luciferase reporter assay. (d) Growth of yeast cells co-transformed with prey and bait on selective medium. proFLC1 contains a bZIP910, while proFLC contains a mutated form of bZIP910 site; (e) Transient expression assay of promoter activity using tobacco cells co-transformed with effector and reporters. Error bars represent ± SE (n = 3); asterisks indicate significant differences (Student’s t-test): ** P < 0.01.