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. 2019 Dec 23;9(1):24. doi: 10.3390/plants9010024

Figure 2.

Figure 2

STY kinases are increased on protein and transcript level after high light (HL) treatment. (A) WT and sty8 sty46 sty17 mutants were probed with STY8 and STY17 specific antisera; 80 mg of fresh weight was used to isolate total soluble protein (=100). Ponceau staining shows equal loading. (B) WT plants were treated with high light for 16 h and total extracted soluble proteins were probed with STY8 or STY17 antisera. Ponceau staining shows equal loading. The immunoblots were quantified and the relative protein levels are shown. Protein levels after normal light (NL) treatment were set to 100% (indicated by dotted line). Statistical significance was determined by Students t-test. P value < 0.05 was considered statistically significant and is indicated by asterisks, n = 3. (C) qRT-PCR was performed with STY8, STY17, and STY46 to analyze the expression level after 16 h HL treatment. Expression levels are shown relative to NL conditions, which were set to 100% for STY8, STY17, and STY46, respectively (indicated by dotted line). Statistical significance was determined by Students t-test. P value < 0.05 was considered statistically significant and is indicated by asterisks, n = 3.