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. 2019 Dec 24;9(1):29. doi: 10.3390/plants9010029

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) SDS-PAGE of HEV 110–660; M. Molecular weight marker (kDa); 1. pEAQ-HT empty vector; 2. Crude extract; 3. SN (soluble protein) after extraction in 3× volume extraction buffer and 13k rpm; 4. Pellet (insoluble part); 5. Positive control (HEV ORF2 452–617 aa recombinant protein) (b) Western blot with anti-HEV ORF2 mAb, the samples are in the same order; (c) Sucrose gradient; M. Molecular weight marker (kDa); 1. SN after pelleting; 2. Dissolved pellet from gradient; 3. 10% sucrose; 4. 20%; 5. 30%; 6. 40%; 7. 50%; 8. 60%; 9. Pellet; 10. Positive control (HEV OF2 452–617 aa recombinant protein).