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(a) Western blot with anti-M2e mAb; M. Molecular weight marker (kDa); 1. Empty pEAQ-HT; 2. TSP extracted from M2 HEV 110–610; 3. Gradient purified M2 HEV 110–610 protein; 4. TSP extracted from HEV 110–610 crude extract; 5. HEV 110–610 after purification; (b). Western blot with anti-M2e mAb of TSP purified from different chimeric constructs; 1. M2 HEV 33–610; 2. M2 HEV 1–610; 3. M2 HEV 110–610 and 4. Empty pEAQ-HT.
