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. 2019 Dec 24;9(1):29. doi: 10.3390/plants9010029

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Relative efficiencies of PVX- and CPMV-based vectors; (a) Coomassie brilliant blue stained gel of 1.5 mg fresh weight (FW) and (b) Western blot of proteins isolated form N. benthamiana plants on day 4 post infiltration and separated by SDS-PAGE and analysed with anti-M2e Ab; (a) 1. Molecular weight marker (kDa); lanes 2–5, total soluble proteins isolated from zones of leaves infiltrated with agrobacteria carrying recombinant vectors pEff-HEV 110–610 (lane 2), pEff-M2 HEV 110–610 (lane 3), pEAQ-HEV 110–610 (lane 4) and pEAQ-M2 HEV 110–610 (lane 5). Lane 6 total soluble proteins isolated from noninfiltrated zone; (b) Lanes 1–3, total soluble proteins isolated from zones of leaves infiltrated with agrobacteria carrying recombinant vector pEff-M2 HEV 110–610 (lane 1), pEAQ-M2 HEV 110–610 (lane 2) and total soluble proteins isolated from noninfiltrated zone (lane 3).