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. 2018 Jul 9;27(4):622–636. doi: 10.1177/0963689718771889

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 2. — Effects of sodium danshensu on proliferation of neural stem cells.

Proliferation of iPSC-derived neural progenitors cultures were analyzed by immunostaining. Representative image showing the Nestin-expressing and BrdU positive cells of neurospheres upon the end of the 4−/4+ RA induction. Sodium danshensu increased the Nestin and BrdU double-labelled cells when compared with those in the vehicle group. Green: Nestin, red: BrdU and blue: Hoechst 33342. n = 5 assays. Data are shown as mean ± SD. ^* P < 0.05 compared with the vehicle group. iPSC: induced pluripotent stem cell; BrdU: 5-bromo-2′-deoxyuridine; RA: retinoic acid; SD: standard deviation.