Table 4.
Flavonoids identified in the green and purple basil varieties by LC-ESI-MS/MS. n: number of peak. RT: retention time. [M+H]+: molecular ion detected in positive mode (m/z: mass/charge). Ref.: reference. (iso): isomer. Glc: glucoside. Mal: malonyl. The RT and fragmentation profile of each compound was verified in all varieties and organs. Quantification of compounds is in Figure 3.
| n. | Compound | RT (min) |
Formula | [M+H]+ (m/z) |
MS2 Fragmentation Profile (m/z) 1 |
Ref. |
|---|---|---|---|---|---|---|
| 18 | Dihydroquercetin glucoside | 7.9 | C21H22O12 | 467.12 | 287.06 (9), 259.06 (80), 231.07 (56), 167.03 (22), 153.02 (86), 149.02 (69), 123.04 (26) | [30,31] |
| 19 | Quercetin diglucoside | 8.7 | C27H30O17 | 627.17 | 303.05 (100) | [30] |
| 20 | Quercetin malonyl Glc Glc | 9.1 | C30H32O20 | 713.16 | 465.10 (10), 303.05 (100) | |
| 21 | Cyanidin diglucoside | 9.2 | C27H31O16 | 611.16 | 287.06 (100) | [30] |
| 22 | Cyanidin rutinoside | 9.5 | C27H31O15 | 595.17 | 287.06 (100) | 2 |
| 23 | Cyanidin-3-Glc-5-(6-Mal)Glc | 9.8 | C30H33O19 | 697.16 | 449.11 (12), 287.06 (100) | [10] |
| 24 | Cyanidin glucoside | 11.1 | C21H21O11 | 449.11 | 287.06 (100) | [30] |
| 25 | Quercetin rutinoside | 11.6 | C27H30O16 | 611.16 | 303.05 (100) | [32] |
| 26 | Quercetin glucoside | 12.0 | C21H20O12 | 465.10 | 303.05 (100) | [30,32] |
| 27 | Anthocyanin A * | 12.1 | C51H53O26 | 1081.28 | 1081.28 (100), 919.23 (79), 449.11 (24), 287.06 (45) | [10,18] |
| 28 | Anthocyanin B (iso1) * | 12.9 | C54H55O29 | 1167.28 | 1167.28 (100), 1005.25 (11), 919.23 (8), 535.11 (15), 287.06 (19) | [10,18] |
| 29 | Anthocyanin B (iso2) * | 13.4 | C54H55O29 | 1167.28 | 1167.28 (100), 1005.23 (57), 449.11 (9), 287.06 (9) | [10,18] |
| 30 | Anthocyanin C * | 13.5 | C51H53O25 | 1065.29 | 1065.29 (99), 903.23 (96), 449.11 (28), 287.06 (62) | [10,18] |
| 31 | Naringenin glucoside | 13.8 | C21H22O10 | 435.13 | 273.08 (100), 153.02 (68), 147.04 (44) | [33] |
| 32 | Apigenin galacturonide | 14.1 | C21H18O11 | 447.09 | 271.06 (100) | [32] |
| 33 | Anthocyanin D (iso1) * | 14.3 | C54H55O28 | 1151.29 | 1151.29 (100), 903.23 (13), 535.11 (24), 287.06 (16) | [10,18] |
| 34 | Cyanidin malonylglucoside | 14.8 | C24H30O14 | 535.11 | 287.06 (100) | [10] |
| 35 | Anthocyanin D (iso2) * | 14.9 | C54H55O28 | 1151.29 | 1151.29 (100), 989.24 (68), 449.11 (11), 287.06 (29) | [10,18] |
1 In brackets are reported the average relative abundances of each ion. 2 Identified by standard.* A: cyanidin-3-(6-p-coumaroyl-6’-caffeoyl)sophoroside-5-glucoside; * B (iso1): cyanidin-3-(6-p-coumaroyl-6’-caffeoyl)sophoroside-5-(6-malonyl)glucoside; *B (iso2): cyanidin-3-(6-p-coumaroyl-malonyl-6’-caffeoyl)sophoroside-5-glucoside; * C: cyanidin-3-(6,6’-di-p-coumaroyl)sophoroside-5-glucoside; * D (iso1): cyanidin-3-(6,6’-di-p-coumaroyl)sophoroside-5-(6-malonyl)glucoside; * D (iso2): cyanidin-3-(6-p-coumaroyl-malonyl-6’-p-coumaroyl)sophoroside-5-glucoside.