Fig.1.

Erythrocyte cytoskeleton proteins co-precipitated with MEC motif constructs. E. coli-expressed and purified MBP-tagged MEC motif constructs from P. falciparum proteins MESA (PFE0040c), PF10_0378, and PFA0675w were incubated with extracts from erythrocyte membranes, precipitated on amylose beads, and subjected to western blotting with antibodies that recognize human 4.1R, ankyrin, spectrin, and actin. MBP-tagged MF4, a fragment from MESA that does not bind to erythrocyte inside out vesicles, was included as a negative control. Molecular weight markers are shown to the left of each blot.