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The experimental design for assay of scavenging O2 −
| AC | ACB | AS | ASB | |
|---|---|---|---|---|
| Tris‐HCl buffer (ml) | 1.0 | 1.0 | 1.0 | 1.0 |
| EDTA solution(ml) | 1.0 | 1.0 | 1.0 | 1.0 |
| Distilled water (ml) | 0.4 | 1.4 | 0 | 1.0 |
| Sample solution(ml) | 0 | 0 | 0.4 | 0.4 |
| Pyrogallol solution (ml) | 1.0 | 0 | 1.0 | 0 |
AC: sample free, with PR. ACB: sample free, PR free. AS: with sample and PR. ASB: with sample, PR free.
