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The experimental design for assay of tyrosinase inhibition
| AC | ACB | AS | ASB | |
|---|---|---|---|---|
| Phosphate buffer (ml) | 0.8 | 0.9 | 0.6 | 0.7 |
| Sample solution (ml) | 0 | 0 | 0.2 | 0.2 |
| Tyrosinase solution (ml) | 0.1 | 0 | 0.1 | 0 |
| L‐Dopa (ml) | 0.1 | 0.1 | 0.1 | 0.1 |
AC: sample free, with TYR. ACB: sample free, TYR free. AS: with sample and TYR. ASB: with sample, TYR free.
