Table 2.
Primers used for the identification of virulent genes in A. hydrophila Ah17
| Gene | Primer sequence (5’−3’) | Reference | Product size (bp) |
|---|---|---|---|
| hly |
Hly F‐ GCCGAGCGCCCAGAAGGTGAGTT Hly R‐ GAGCGGCTGGATGCGGTTGT |
Wang et al. (2003) | 130 |
| act |
Act F‐ GAGAAGGTGACCACCAAGAACA Act R ‐ AACTGACATCGGCCTTGAACTC |
Kingombe et al. (1999) | 232 |
| aer |
Aer F‐ CACAGCCAATATGTCGGTGAAG Aer R‐ GTCACCTTCTCGCTCAGGC |
Singh, Rathore, Kapoor, Mishra, and Lakra (2008) | 326 |
| ast |
Ast F‐ TCTCCATGCTTCCCTTCCACT Ast R‐ GTGTAGGGATTGAAGAAGCCG |
Sen and Rodgers (2004) | 331 |
| ahyB |
Ela F‐ ACACGGTCAAGGAGATCAAC Ela R‐ CGCTGGTGTTGGCCAGCAGG |
Sen and Rodgers (2004) | 513 |
| lip |
Lip F‐ ATCTTCTCCGACTGGTTCGG Lip R‐ CCGTGCCAGGACTGGGTCTT |
Sen and Rodgers (2004) | 382 |