Table 3.
Nanopore sequencing metrics for DENV RNA standards, using the single-plex (2500 bp amplicon) approach
Serotype | Genotype | Reads Passing QC | 20X Coding-Region Coverage (%) | Nucleotide Consensus Similarity (%) |
---|---|---|---|---|
1 | I | 31,601 | 100 | 99.99 |
2 | IV | 8274 | 100 | 99.93 |
3 | V | 14,300 | 100 | 99.98 |
4 | I | 31,486 | 100 | 99.96 |