Table 4.
Bacterial concentration and leukocyte counts performed with the immunohistochemistry slides of the mammary tissue from all the different experimental groups taken at the end of the immunological challenge (day 7 experimental post-infection)
| Group | |||||||
|---|---|---|---|---|---|---|---|
| Control | ST96 | ST121 | P-value | ||||
| No. of animals | 6 | 9 | 9 | ||||
| Total bacteria (log10[CFU]/g) | − 2.00 | ± 0.80a | 1.04 | ± 0.65b | 6.23 | ± 0.65c | 0.0001 |
| Leukocytes counts (cells/mm2) | |||||||
| CD3+ | |||||||
| At nipple | 27.5 | ± 33.5a | 97.5 | ± 27.4ab | 163.5 | ± 27.4b | 0.0176 |
| 1 cm from nipple | 18.13 | ± 32.57 | 59.57 | ± 26.66 | 97.51 | ± 26.66 | 0.1920 |
| RAM11+ | |||||||
| At nipple | 84.1 | ± 61.2a | 219.9 | ± 50.1a | 435.5 | ± 50.1b | 0.0007 |
| 1 cm from nipple | 29.4 | ± 58.1a | 99.5 | ± 47.5ab | 218.9 | ± 47.5b | 0.0519 |
| CD79+ | |||||||
| At nipple | 82.5 | ± 38.5 | 141.2 | ± 31.5 | 147.0 | ± 31.5 | 0.3967 |
| 1 cm from nipple | 87.75 | ± 23.75 | 91.63 | ± 19.51 | 66.43 | ± 19.51 | 0.6345 |
| IgG+ | |||||||
| At nipple | 339.6 | ± 32.3 | 293.1 | ± 26.4 | 343.8 | ± 26.4 | 0.3599 |
| 1 cm from nipple | 336.3 | ± 32.5b | 255.3 | ± 26.6ab | 210.0 | ± 26.6a | 0.0624 |
CFU colony-forming units.
abcMeans in a row not sharing superscripts significantly differ at P < 0.05.