Fig. 4.

Gene mutations and variation spectrums in the chromosome of AA-MSCs and HD-MSCs. a–c There was no significant difference in the subtypes of single nucleotide polymorphisms (SNPs), SNP distribution and insertion/deletion (INDEL) distribution between AA-MSCs and HD-MSCs. d Different somatic variations and gene fusion events were analyzed by Circos software. And their loci regional distribution was also further confirmed. e, f Differential variable shear events (DVSE) including skipped exon (SE), retained intron (RI), mutually exclusive exon (MXE), alternative 3′ splicing site (A3SS), and alternative 5′ splicing site (A5SS) were analyzed. And SE accounts for 58% of DVSE. g, h The GO analysis showed differential spliceosomes (DSs) were relevant to a series of biological process. h Enriched DSs were associated with different signaling pathways