Figure 5.

Quantitative PCR analysis of two DcaAG genes at different tissues (A,B) and floral organs (C,D) in single and double flower phenotype carnations. In different tissues, the expression of DcaAGa (A) and DcaAGb (B) were obviously different in the flower bud, and no significant difference was detected in the root, stem and leaf between two flower phenotype carnations. In floral organs, the expression of DcaAGa (C) and DcaAGb (D) were exclusively expressed in the stamen and carpel, and low expression levels were detected in the sepal and petal. DcaAGa (C) and DcaAGb (D) showed significant differential expression in the carpel and stamen between two flower phenotype carnations, respectively. ** indicates p < 0.01 by student’s t-test.