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. 2020 Jan 13;12(1):46. doi: 10.3390/toxins12010046

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of T-2 toxin treatment on cellular metabolic activity of chicken-derived primary hepatocyte mono- and hepatocyte—nonparenchymal cell co-cultures with the cell ratio of 6:1 (hepatocytes to nonparenchymal cells). Cells after 24 h culturing were treated with different concentrations of T-2 toxin for 8 (A) or 24 h (B). “T0” refers to control group (without T-2 toxin treatment); “T10” refers to 10 nmol/L T-2 toxin treatment; “T100” refers to 100 nmol/L T-2 toxin treatment; T1000 refers to 1000 nmol/L T-2 toxin treatment. Cellular metabolic activity was measured by Cell Counting Kit-8 (CCK-8) assay. Relative absorbances were calculated by considering the mean value of T0 hepatocyte mono-cultures as 1. Results are expressed as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. n = 3/group.

Effects of T-2 toxin treatment on cellular metabolic activity of chicken-derived primary hepatocyte mono- and hepatocyte—nonparenchymal cell co-cultures with the cell ratio of 6:1 (hepatocytes to nonparenchymal cells). Cells after 24 h culturing were treated with different concentrations of T-2 toxin for 8 (A) or 24 h (B). “T0” refers to control group (without T-2 toxin treatment); “T10” refers to 10 nmol/L T-2 toxin treatment; “T100” refers to 100 nmol/L T-2 toxin treatment; T1000 refers to 1000 nmol/L T-2 toxin treatment. Cellular metabolic activity was measured by Cell Counting Kit-8 (CCK-8) assay. Relative absorbances were calculated by considering the mean value of T0 hepatocyte mono-cultures as 1. Results are expressed as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. n = 3/group.