Fig. 3.

Cleavage of the γ-glutamyl bond in N- and S-linked glutathione adducts under physiological conditions. a) Loss of Glu from S-Me-GSH (open circle) and loss of Glu from γ-Glu(DHA)Gly PE crosslink (closed circle) both incubated at pH 7.0, 37°C. The loss of Glu was monitored at 216nm by HLPC. b) Mass spectrum of the (DHA)Gly - PE product generated after incubation of γ-Glu(DHA)Gly - PE. MI = molecular ion.