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. Author manuscript; available in PMC: 2020 Feb 14.
Published in final edited form as: J Mol Biol. 2018 Jul 12;430(18 Pt B):3170–3189. doi: 10.1016/j.jmb.2018.07.008

Fig. 3. Effects of bcsG variants on the expression of the cellulose synthase auxiliary subunits BcsZ and BcsC.

Fig. 3.

A. Production of the cellulase BcsZ in the ΔbcsG mutants of the cellulose synthase-positive strain MAE97. MAE97 bcsZ deletion strains with pBAD30 vector control (ΔbcsZ-VC) and with overexpressed BcsZ (ΔbcsZ-pBcsZ) were used as negative and positive controls, respectively. Detection of BcsZ production was by Western blot using a rabbit anti-BcsZ antibody.

B. Production of the putative outer membrane pore BcsC in S. typhimurium wild type and bcsG derivatives. Wild-type S. typhimurium UMR1 with BcsC-3xFLAG and its ΔbcsG mutant were complemented by the same plasmids as in panel A with the addition of the second truncated variant, pBcsG1–165. Detection of the 132.7 kDa BcsC-3xFLAG was by Western blot using a mouse anti-FLAG-tag antibody. UMR1 BcsC-3xFLAG with pBAD30 (vector control, left lane) and UMR1 without the FLAG tag (right lane) were used as positive and negative controls, respectively.

Cells were grown on salt-free LB agar plates for 24 h at either 28 °C (A, top lane) or 37 °C (A, bottom lane), or for 16 h at 28 °C (panel B).