Fig. 5. Enzymatic activity of BcsG.
Hydrolysis of NBD-phosphatidylethanolamine (PE) by BcsG, but not the BcsGS278A variant. B. NBD-phosphatidylglycerol (PG) is not hydrolyzed by BcsG. The reaction was carried out in 300 μl of 10 mM sodium succinate pH 6.5/50 mM NaCl, 20 mM MnCl2 with 4.2 ng of purified NBD-PE or NBD-PG lipid and 400 μg MBP-BcsG fusion protein (or 2.5 units of phospholipase C from Bacillus cereus used as positive control) at 37 °C for 3 h. Substrates NBD-PE and NBD-PG are shown below the TLC plates. Each experiment was performed twice.