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. Author manuscript; available in PMC: 2020 Feb 14.
Published in final edited form as: Eur J Immunol. 2011 Aug 17;41(10):2977–2986. doi: 10.1002/eji.201141639

Figure 4. Three-component treatment affects tumor-infiltrated T cell profile.

Figure 4.

C57BL/6 mice (n=6 per group) were injected s.c. in the right flank with 5×10e4 TC-1 cells. On day 7, CPM or PBS was injected. On day 8 and 15 mice were injected with vaccine+CT-011, vaccine alone, CT-011 alone, PBS or IgG. On day 21 mice were sacrificed, and tumor infiltrated T cells were analyzed in tumor homogenates by flow cytometry. The absolute numbers of infiltrated cells were standardized per 10e6 of total tumor cells and presented as mean values ± SD for (A) CD8+, (B) CD4+FoxP3− and (C) CD4+FoxP3+ T cells. The mean values of CD8+ T cell ratios to Treg (D) and CD4+FoxP3− to Treg cells (E) ± SD are shown. *P<0.05, **P<0.01 and ***P<0.001. Similar results were obtained from two independent experiments.