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NOX5 silencing inhibited the expression of key proteins in TLR4/NF-κB signaling pathway in HMCs induced by HG. The expression of TLR4, MyD88, p-NF-κB p65 and NF-κB p65 were determined by western blotting. ### P<0.001 versus NG; ** P<0.01 versus HG+siRNA-NC. NOX5 – NADPH oxidase 5; HG – high glucose; HMCs – human glomerular mesangial cells; TLR4 – toll-like receptor 4; p-NF-κB p65 – phosphor-NF-κB p65.
