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. 2020 Feb 7;8:49. doi: 10.3389/fbioe.2020.00049

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Copyright © 2020 Lobner, Wachernig, Gudipati, Mayrhofer, Salzer, Lehner, Huppa and Kunert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding of CD19-AD2 to CD19-directed CAR-T cells. (A) CAR-T cells were incubated with various concentrations of CD19-AD2-AF555 (three representative concentrations are shown) and subjected to flow cytometry. The filled gray histogram represents the incubation of mock-T cells with the highest concentration of CD19-AD2-AF555. One experiment representative of four independent experiments is shown. (B) CD19-AD2 binding isotherm was generated by fitting the data points obtained from titrating CD19-AD2-AF555 to CAR-T cells to a one-set-of-sites binding model (K_D = 25.0 ± 4.9 nM, n = 4).