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. 2020 Feb 7;7:46. doi: 10.3389/fvets.2020.00046

Figure 2.

Figure 2

Antigen-specific immune response of broiler chickens reared in the HS condition (A). The experimental schedule is summarized. All birds were maintained in either TN or HS condition throughout the 14-days experimental period. To assess antigen-specific immune responses of heat-stressed chickens, broiler chickens under the TN (TN-BSA) and HS (HS-BSA) conditions were immunized intramuscularly with 10 μg of BSA on days 3 and 10 of the experiment. As a control, other broiler chickens, maintained under the TN and HS conditions, were immunized intramuscularly with phosphate-buffered saline on days 3 and 10 of the experiment. Blood samples were collected from the wing veins of all chickens 4 days after initial and booster immunization (B,C). Total plasma IgY, IgM, and IgA levels (B) and BSA-specific IgY, IgM, and IgA responses (C) were analyzed by ELISA. Three experiments were conducted and a representative result was shown. The data are presented as means ± SE (n = 10–12/group). *p < 0.05 compared between TN-BSA and HS-BSA (unpaired two-tailed Student's t-test). Clear, TN; gray, HS; blue; TN-BSA; purple, HS-BSA.