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. 2020 Feb 10;13:313–323. doi: 10.2147/DMSO.S242136

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© 2020 Wen et al.

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GSDME mediates secondary necrosis and fibrosis. (A–C) GSDME knock-down was verified using RT-qPCR and Western blot. (D and E) The percentage of cells dead by secondary necrosis decreases in GSDME shRNA transfected cells, as analyzed by flow cytometry. (F) Cell death is alleviated by GSDME knock-down as assessed by LDH release. (G–I) Immunoblotting reveals elevation of collagen I and fibronectin expression are inhibited in cells transfected with GSDME shRNA. Densitometry of the data in H and I. Results are representative of at least three independent experiments. Data from at least three independent experiments are shown as the means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Abbreviations: NG, normal glucose; HG, high glucose; NT shRNA, non-target shRNA; NS, not significant.