Table 2.

Steady‐state kinetic parameters for tagged and cleaved enzymes toward chloramphenicol (Cm)

Enzyme	K m for Cm (mM)	k cat (s−1)	k cat/K m (M−1 s−1)
Tagged protein
VcCAT	0.764 ± 0.037	63	8.25 × 104
VvCAT	1.02 ± 0.03	43	4.22 × 104
AfCAT	0.803 ± 0.064	21	2.62 × 104
Cleaved protein
VcCAT	0.939 ± 0.056	95	1.01 × 105
VvCAT	0.913 ± 0.059	78	8.54 × 104
AfCAT	0.659 ± 0.082	31	4.70 × 104