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. 2019 Nov 20;29(3):686–694. doi: 10.1002/pro.3766

Table 1.

Steady‐state kinetic parameters of fungal IMPDHs

Enzyme

K _m IMP (μM)

K _m NAD⁺ (μM)

K _ii NAD⁺ (mM)

k _cat (s⁻¹)

K _ii MPA versus IMP (nM)

K _ii MPA versus NAD⁺ (nM)

^D V

^{D_{2} O} V

AnIMPDH

15 ± 1

120 ± 8

1.5 ± 0.1

1.9 ± 0.1

23 ± 1

13 ± 2

1.1 ± 0.1

2.3 ± 0.1

AnIMPDH‐A267S

15 ± 1

190 ± 20

2.2 ± 0.2

1.9 ± 0.1

150 ± 30

77 ± 12

1.0 ± 0.1

1.8 ± 0.1

PbIMPDHA

93 ± 5

400 ± 40

3.0 ± 0.4

2.7 ± 0.1

86 ± 18

47 ± 7

1.4 ± 0.1

1.5 ± 0.1

PbIMPDHA‐S267A

79 ± 6

330 ± 40

2.6 ± 0.3

1.6 ± 0.1

35 ± 6

21 ± 3

1.4 ± 0.1

1.4 ± 0.3