. 2019 Nov 20;29(3):686–694. doi: 10.1002/pro.3766

Table 2.

Effect of increasing E‐XMP* on MPA inhibition. $^{D_{2} O} K_{i app}$ = K _iapp(H₂O)/K _iapp(D₂O). ^APAD K _iapp = K _iapp(NAD⁺)/K _iapp(APAD⁺)

Enzyme

K _iapp(H₂O)^a (nM)

K _iapp(D₂O) (nM)

^{D_{2} O} K_{i app}

K _iapp(NAD⁺)^a (nM)

K _iapp(APAD⁺) (nM)

^APAD K _iapp

AnIMPDH

16 ± 2

8 ± 1

2.0 ± 0.2

24 ± 3

8.7 ± 0.8

2.8 ± 0.2

AnIMPDH‐A267S

78 ± 6

51 ± 9

1.5 ± 0.2

73 ± 3

49 ± 2

1.5 ± 0.1

PbIMPDHA

73 ± 4

27 ± 2

2.7 ± 0.1

110 ± 5

56 ± 2

1.6 ± 0.1

PbIMPDHA‐S267A

28 ± 2

11 ± 6

2.5 ± 0.6

19 ± 2

12 ± 1

1.6 ± 0.1

Independent determinations for D₂O and APAD⁺ experiments.