FIG 5.
Impact of apical E. faecalis supernatants on basolateral GLP-1 degradation. (A) Schematic of experimental setup. T84 epithelial cells seeded onto a transwell insert, with E. faecalis supernatants added to the upper chamber and GLP-1 in cell culture medium (DMEM–F-12) to the lower chamber (schematic not drawn to scale). (B) After incubation, GelE-producing supernatants decrease the integrity of a T84 epithelial cell layer while degrading GLP-1 on the basolateral side of the epithelium, compared to the DMEM–F-12 T84 cell culture medium control. Data were obtained from four independent experiments and are expressed as the mean ± SD. For TEER: 30054A **, P = 0.0022; OG1RF *, P = 0.0439; ΔgelE **, P = 0.0045; S613 **, P = 0.0084. For GLP-1: 30054A and OG1RF ****, P < 0.0001 with two-way ANOVA. GM17 (mc), bacterial culture medium control.