Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | w; elav-Gal4 (III) | C. Doe University of Oregon | ||
| Genetic reagent (D. melanogaster) | yw; wg(Sp)/CyO;Dr(Mio)/TM3, Sb | E. Ferguson, University of Chicago | ||
| Genetic reagent (D. melanogaster) | yw; ppk-CD4-tdtomato (II) | Bloomington Drosophila Stock Center (BDSC) | Stock number 35844; FBst0035844; RRID:BDSC_35844 |
Fly base genotype: w1118; P{ppk-CD4-tdTom}4a |
| Genetic reagent (D. melanogaster) | w; D42-Gal4 (III) | BDSC | Stock number 8816; FBst0008816; RRID:BDSC_8816 |
Fly base genotype: w*; P{GawB}D42 |
| Genetic reagent (D. melanogaster) | y sc v; UAS-Trc-RNAi | BDSC | Stock number 41591; FBst0041591; RRID:BDSC_41591 |
Fly base genotype y1 sc*v1 sev21; P{TRiP.GL01127}attP2 |
| Genetic reagent (D. melanogaster) | y sc v; UAS-Pav-RNAi | BDSC | Stock number 42573; FBst0042573; RRID:BDSC_42573 |
Fly base genotype: y1 v1; P{TRiP.HMJ02232}attP40 |
| Genetic reagent (D. melanogaster) | y sc v; UAS-Fry-RNAi | BDSC | Stock number 60103; FBst0060103; RRID:BDSC_60103 |
Fly Base Genotype: y1 sc*v1 sev21; P{TRiP.HMC05097}attP40 |
| Genetic reagent (D. melanogaster) | w; UASp-tdMaple3-alpha tubulin 84b | This lab | Described in Lu et al. (2016) Generated by injection of pUASp tdMaple3-alpha tubulin 84b. A second chromosome insertion was used in this study. | |
| Genetic reagent (D. melanogaster) | yw; UASp-tdMaple3-alpha tubulin 84b; UAS-Trc-RNAi | This lab | Generated using an insertion on the second chromosome | |
| Transfected construct (D. melanogaster) | pMT EOS tubulin | This lab | Described in Barlan et al. (2013). For S2 cell expression of EOS tubulin. | |
| Transfected construct (D. melanogaster) | pMT-BFP Trc WT | This lab | S2 cell expression of Trc. Generated from pUASt-Trc WT using EcoRI and NotI, a kind gift from P. Adler. | |
| Transfected construct (D. melanogaster) | pMT-BFP Trc CA (T253E) | This lab | S2 cell expression of Trc. Generated from pUASt-Trc T253E (a kind gift from P. Adler) and pMT vector using EcoRI and NotI. | |
| Transfected construct (D. melanogaster) | pMT-BFP Trc kinase dead (K122A) | This lab | S2 cell expression of Trc. Generated from pUASt-Trc K122A (a kind gift from P. Adler) and pMT vector using EcoRI and NotI. | |
| Transfected construct (D. melanogaster) | pMT-GFP Pavarotti | This lab | S2 cell expression. Generated from pMT-BFP Pavarotti (Del Castillo et al., 2015) | |
| Transfected construct (D. melanogaster) | pMT-GFP Pavarotti S745A | This lab | S2 cell expression. Generated by site directed mutagenesis of WT. | |
| transfected construct (D. melanogaster) | pEGFP Pavarotti WT | This lab | Mammalian expression. Pavarotti constructs were subcloned into pEGFP-C1 using EcoRI and SalI. | |
| Transfected construct (D. melanogaster) | pEGFP Pavarotti S745A | This lab | Mammalian expression. Pavarotti constructs were subcloned into pEGFP-C1 using EcoRI and SalI. | |
| transfected construct (D. melanogaster) | pcDNA Trc T253E | This lab | Mammalian expression. Trc T253E was subcloned into pcDNA 3.1+ using HindIII and NotI. | |
| Transfected construct (D. melanogaster) | pMT mCherry tubulin | This lab | For S2 cell expression. Described in del Castillo et al. (2015) | |
| Cell line (D. melanogaster) | S2 cells | DGRC | FlyBase Report: FBtc0000006 | Cell line maintained in this lab. |
| Cell line (Homo-sapiens) | HEK 293 FT | ATCC | RRID:CVCL_0045; ATCC: CRL-1573 | Cell line maintained in this lab. |
| Antibody | Single chain anti-GFP, GFP-Trap-M | Chromotek | ||
| Antibody | Anti Pavarotti (Rabbit polyclonal) | Scholey lab | Western blot 1:1000 | |
| Antibody | Anti Trc (Rabbit polyclonal) | Emoto lab | Western blot 1:1000 | |
| Antibody | Anti Tubulin (Rabbit polyclonal) | This lab | Affinity purified from immunized rabbit serum. Western blot 1:1000 | |
| Antibody | Anti phospho-Pavarotti S710 (Rabbit polyclonal) | Mishima lab | Western blot 1:1000 | |
| Antibody | Anti GFP (Rabbit polyclonal) | This lab | Affinity purified from immunized rabbit serum. Western blot 1:1000 | |
| Antibody | Anti Furry (Rabbit polyclonal) | Adler lab | Immunofluorescence 1:250 | |
| Antibody | Anti Hsc 70 (Goat polyclonal) | Santa cruz | K-19, RRID:AB_2120291 | Western blot 1:100 |
| Antibody | Anti 14-3-3ζ (Rabbit polyclonal) | Proteintech group | cat no. 14503–1-AP; RRID:AB_2218096 | Western blot 1:250 |
| Antibody | HRP conjugated Anti Rabbit | Jackson | Western blot 1:10,000 | |
| Antibody | HRP conjugated Anti Goat | Jackson | Western blot 1:10,000 |