Extended Data Figure 1. Characterization of HSPC (Mds1^GFP/+) mice demonstrates normal hematopoiesis, HSC frequency, cell cycle and stimuli recovery response.

a, Targeting strategy for the generation of Mds1^GFP//+ mice. b, 8–12 week old Mds1^GFP/+ mice (triangles, N=9 mice) displayed similar bone marrow cellularity as control mice (Mds1^+/+) (circles, N=7 mice). Middle line represents mean. Error bars demonstrate SD. c, 8–12 week old Mds1^GFP/+ mice (N=14 mice) displayed similar peripheral blood parameters as Mds1^+/+ control mice (N=11 mice). Middle line demonstrates mean. Error bars demonstrate SD. d, 8–12 week old Mds1^GFP/+ mice (triangles, N=7 mice) displayed similar CD150⁺CD48^-LSK (LT-HSC), CD150^-CD48^-LSK (ST-HSC) and CD150^-CD48⁺LSK (MPP) frequencies as control (circles, Mds1^+/+) mice (N=4 mice). Middle line represents mean. Error bars demonstrate SD. e, Cell cycle analysis of SLAM cells from Mds1^GFP/+ (N=3 mice) and wild type (Mds1^+/+) mice (N=2 mice) in native conditions. Indicated value per gate represents mean ± standard deviation. f, Dynamics of white blood cells (WBC), lymphocytes (LY) and red blood cells (RBC) recovery upon 5-fluorouracil treatment in Mds1^GFP/+ and control (Mds1^+/+) mice. Middle value represents mean. Error bars represent standard deviation. N=4 mice.