Table 3.

Sequences of Target Gene primers for SYBR Green qRT-PCR

Target genes	Accession number	primer length	Tm	Sequence
ZEB1 Forward	NM-001174096	21	57	5a AGCCAAATGGAAATCAGGATG 3′
ZEB1 Reverse		20	58	5E TTTGGGCGGTGTAGAATCAG 3′
ZEB2 Foeward	NM-014795	20	58	5E AGAAAATGACCTGCCACCTG 3′
ZEB2 Reverse		20	60	5o CTTCATTCTTCTCGTGGCGG 3′
E-cadherin Forward	NM-004360	20	60	5E ACCGAGAGAGTTTCCCTACG 3′
E-cadherin Reverse		21	61	5- CGGAGGATTATCGTTGGTGTC 3′
GAPDH Forward	NM-001289745	21	63	5- TCCACCACCCTGTTGCTGTAG 3′
GAPDH Reverse		21	63	5A ACACCCACTCCTCCACCTTTG 3′