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. 2020 Feb 14;10:2675. doi: 10.1038/s41598-020-59465-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SE/HPLC-DAD chromatograms for the samples of ziv-AFL (Zaltrap 25 mg/mL) subjected to physical stress. (A) Ziv-AFL Temperature stress 60 °C: (1) ziv-AFL control, (2) 1 hour of exposure, (3) 2 hours of exposure and (4) 3 hours of exposure. (B) Ziv-AFL light stress: (1) Ziv-AFL control and (2) 12 hours of exposure. (C) Ziv-AFL freeze-thaw stress: (1) Ziv-AFL control, (2) 1 freeze-thaw cycle and (3) 2 freeze-thaw cycles. The retention times represented in the SEC chromatograms are the mean of three chromatograms.