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. 2020 Feb 14;10:2668. doi: 10.1038/s41598-020-59404-6

Figure 3.

Figure 3

TEM observations showing the impact of PEF and mechanical compressions on 7-day stressed C. reinhardtii cells. (a) Control; the integrity of the membrane and cell wall (left image) and chloroplast (right image) can be observed, (b) cells submitted to reversible electroporation (5.5 kV∙cm−1, 5 µs); both images show the structural impact on the chloroplasts, (c) cells submitted to reversible (left image) and irreversible (right image) electroporation (7 kV∙cm−1, 5 µs), (d) cells submitted to irreversible electroporation; a pore on the membrane can be seen, (e) cells submitted to the pretreatment combining reversible electroporation and mechanical compressions. CW: Cell wall; L: lipid droplets; S: Starch; Chl: Chloroplast; Mb: Plasma membrane; EM: Extracellular Medium34.