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. 2020 Feb 14;10:2672. doi: 10.1038/s41598-020-59354-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of the study. Time-mated rat dams were exposed to PFHxS with or without a mixture of environmentally relevant endocrine disrupting chemicals from gestation day 7 through to postnatal day 22. Blood was taken from dams and pups for thyroid hormone assessments and organs were excised, weighed and stored for further analysis on PD 16/17 and 22. A subset of offspring was weaned (PD 22) and used for testing of motor activity levels at three ages, and learning and memory in the radial arm maze (4–5 months of age for the females and 8–9 months for the males). The litter was used as statistical unit for all analyses. PFHxS: Perfluorohexane sulfonate, GD: Gestation day, PD: Postnatal day.