Table 1.
CryoEM data collection, refinement, and validation statistics.
| ImmZIKV-Fab DV62.6 complex [SPA] (EMDB-0932) (PDB 6LNT) | ImmZIKV (EMDB-0933) (PDB 6LNU) | ImmZIKV-Fab DV62.5 complex [StA] (EMDB-0933) | |
|---|---|---|---|
| Data collection and processing | |||
| Magnification | 47,000 | 59,000 | 47,000 |
| Voltage (kV) | 300 | 300 | 300 |
| Electron exposure (e–/Å2) | 18 | 18 | 90 |
| Defocus range (μm) | −0.5 to −3.5 | −0.5 to −3.5 | −3 to −4 |
| Pixel size (Å) | 1.71 | 1.34 | 1.71 |
| Symmetry imposed | I | I | C1 |
| Initial particle images (no.) | 84,924 | 79,701 | 1934 |
| Final particle images (no.) | 19,295 | 7,922 | 1152 |
| Map resolution (Å) | 8 | 9 | 13 |
| FSC threshold 0.143 | |||
| Map resolution range (Å) | ≥8 | ≥9 | ≥13 |
| Refinement | |||
| Initial model used (PDB code) | 4B03, 5AZE, 4JZN | 4B03 | NA |
|
Model resolution (Å) FSC threshold 0.5 |
9.1 | 9.4 | NA |
| Model resolution range (Å) | ≥9.1 | ≥9.4 | NA |
| Map sharpening B factor (Å2) | −900 | −912 | |
| Model composition | NA | ||
| Non-hydrogen atoms | 2642 | 1998 | |
| Protein residues | 2642 | 1998 | |
| Ligands | - | - | |
| B factors (Å2) | NA | NA | NA |
| Protein | |||
| Ligand | |||
| R.m.s. deviations | NA | NA | NA |
| Bond lengths (Å) | |||
| Bond angles (°) | |||
| Validation | NA | ||
| MolProbity score | 2.15 | 1.94 | |
| Clashscore | 2.27 | 1.00 | |
| Poor rotamers (%) | NA | NA | |
| Ramachandran plot | NA | NA | NA |
| Favored (%) | |||
| Allowed (%) | |||
| Disallowed (%) | |||
SPA single-particle analysis, StA subtomogram averaging