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. 2020 Feb 14;11:912. doi: 10.1038/s41467-020-14628-y

Fig. 3. Normal cilia structure and planar polarity of ependymal cells in Dgcr8+/− mice.

Fig. 3

a–d Representative TEM images of motile cilia in ependymal cells of WT and Dgcr8+/− mice. Cilia are transected at the levels of the axoneme (a, b) or basal body (c, d). e–h Representative SEM images of motile cilia in ependymal cells of WT and Dgcr8+/− mice. i–l Confocal images of basal body patch position as an anatomical indicator of ependymal planar polarity in the anterior dorsal (i, j) and anterior ventral (k, l) LV walls of WT and Dgcr8+/− mice. Whole-mount brains were stained with antibodies against β-catenin (green, intercellular junctions) and γ-tubulin (red, basal bodies). m–o Mean ciliary length (m), ciliary number in a bundle (n), and distance of the basal body (BB) displacement from the center of an ependymal cell (o) in WT and Dgcr8+/− mice. In m, 105 cilia in 3 mice of each genotype were analyzed using the Mann–Whitney rank-sum test (U = 5156, p = 0.42). In n, 72 cilia in three mice of each genotype were analyzed in the same manner (U = 2379, p = 0.39). In o, 309 cells in 3 WT mice and 305 cells in 3 Dgcr8+/− mice were analyzed using the Shapiro–Wilk normality test (U = 44,160, p = 0.18). p Distribution of the basal body patch is plotted on a polar histogram. Average angles of the individual vectors in each imaged section were normalized to 0°, and distributions of the angles were compared in WT (235 cells, three mice) and Dgcr8+/− (135 cells, three mice) animals. The data were analyzed using Watson’s U2 two-sample test of homogeneity (t = 0.13, p = 0.13). Source data are provided as a Source Data file.