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. 2020 Jan 28;412(6):1263–1275. doi: 10.1007/s00216-019-02296-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — MALDI MSI images of FFPE tissues using the optimized SOP. Shown are MALDI MSI images of mouse organs using the optimized SOP and overlaid on the tissue scan. a Kidney, 184 (m/z) red, 828 (m/z) green, 932 (m/z) blue. *Grid size 50 μm. b Heart, 657 (m/z) green, 445 (m/z) red. *Grid size 50 μm. c Aorta, 864 (m/z) red and 237 (m/z) blue. *Grid size 20 μm