Table 1.
Cryo-EM data collection, model refinement and validation statistics.
| FPR2-Gi1-scFV16-WKY (EMDB- EMD-20126) (PDB 6OMM) | |
|---|---|
| Data collection and processing | |
| Magnification | 130,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | ~67 |
| Defocus range (μm) | 0.6-2.5 |
| Pixel size (Å) | 1.029 |
| Symmetry imposed | None |
| Initial particle images (no.) | 1,231,594 |
| Final particle images (no.) | 203,133 |
| Map resolution (Å) | 3.17 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 50-3.2 |
| Refinement | |
| Initial model used (PDB code) | 6DDE |
| Model resolution (Å) | 3.17 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | 50-3.2 |
| Map sharpening B factor (Å2) | -117.3 |
| Model composition | |
| Non-hydrogen atoms | 9134 |
| Protein residues | 1142 |
| Ligands | 8 |
| B factors (Å2) | |
| Protein | 74.7 |
| Ligand | 74.0 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 1.153 |
| Validation | |
| MolProbity score | 1.48 |
| Clashscore | 4.74 |
| Poor rotamers (%) | 0.0 |
| Ramachandran plot | |
| Favored (%) | 96.44 |
| Allowed (%) | 3.56 |
| Disallowed (%) | 0.0 |