Figure 2.

CCL2 deficiency delays wound re-epithelialisation compared to MSC-WT. (a) Histological samples taken at d-10 post wounding were stained with H&E. Images of stained tissue sections were taken at 4×. Black arrows indicate migrating front of Keratinocytes; insets show magnified image of migrating front. Scale bar, 100 μm. (b) Left- Quantification of unepithelialised gap demonstrated significantly reduced unepithelialised wound in MSC-WT treated wounds compared to both control and MSC-KO (n ≥ 8 ns, not significant *p < 0.05, **p < 0.01). (b) Right - Granulation area was measured using ImageJ software indicated significantly reduced area in MSC-WT treated wounds compared to control suggestive of reduced inflammatory response and increased wound maturity (n ≥ 7 ns, not significant, *p < 0.05).