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. 2020 Feb 14;10:2633. doi: 10.1038/s41598-020-59164-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of rpoB cross contamination risk experimental set-up and results. (A) Configuration of the environmental exposure experiment within a Biosafety level 2 cabinet. Three microcentrifuge tubes were open throughout each batched extraction procedure and three remained closed [median (IQR) extractions per batch 17.5 (10.5–27.5)]. No exposed tubes were FT rpoB-positive. In parallel to evaluate if, in an absolute worst case scenario, rpoB cross-contamination was probable, dCE from a (B) Ultra or (C) Xpert done on a drug-resistant strain was added to a drug-susceptible strain and the resultant mixture tested by Ultra. When samples of DS-TB contained CE at higher concentrations (undiluted, 10⁻³), false-resistant (solid black circles) or indeterminate rifampicin resistance (grey circles) are seen. All samples containing CE dilutions beyond 10⁻⁶ showed true rifampicin susceptibility (white circles). Error bars represent C_Tmin values for each dilution. Some images were obtained from the Noun Project: microcentrifuge tube (without changes), Anthony Ledoux, https://thenounproject.com/term/eppendorf/1699532/; spray bottle (without changes), John Winowiecki, https://thenounproject.com/search/?q=spray%20bottle&i=2236898; sharps container, Juicy Fish (with changes), https://thenounproject.com/term/hospital-waste-bin/2450390/; needle (without changes), Creative Mania; https://thenounproject.com/search/?q=injection&creator=2251916&i=2409865.