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. 2020 Feb 14;10:2633. doi: 10.1038/s41598-020-59164-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Receiver operation area under the curve of actionable vs. non-actionable results of MTBDRsl on Ultra diamond cartridge extract done on DR-TB clinical sputa to determine a C_Tmin threshold at which this approach is not feasible. MTBDRsl yields actionable results on cartridge extract from Ultra at a C_Tmin threshold of <25.4 with a sensitivity of 97% (87.1–99.9; 95% CI) and specificity of 100% (54.9–100; 95% CI) respectively.