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. 2020 Feb 14;10:2634. doi: 10.1038/s41598-020-59462-w

Figure 3.

Figure 3

Effect of osmotic pressure on U251 and U87 in vitro invasion potential. (a) Cell invasion through Matrigel-coated Transwells was determined in control (285 mOSmol/kg) or hyperosmotic (440 mOsmol/kg) media. Representative micrographs with crystal violet-stained cells are shown. Quantitation of invaded cells is shown as percent of normo-osmotic control. Results are expressed as mean ± SEM of n = 3 independent experiments, *p < 0.05, **p < 0.01, unpaired Student t test. (b) mRNA expression of EMT markers in U87 U251 and 081024 cells after 48 h of exposure to control (285 mOsmol/kg for adherent cells, 335 mOsmol/kg for oncospheres) hyper or hypoosmotic stress. All results are expressed relative to cells incubated in normo-osmotic medium as mean ± SEM of n = 3 independent experiments, *p < 0.05, **p < 0.01, ****p < 0.0001, one way ANOVA analysis with Dunnett’s multiple comparisons test.