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. 2020 Feb 14;10:2634. doi: 10.1038/s41598-020-59462-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of hydrostatic pressure on uPA production by GBM adherent and neurosphere cells. (a) Conditioned media of cells exposed to hydrostatic pressure for 48 h were analysed by casein plasminogen zymography. (b) Densitometric quantitation of the 47 KD and 51 KD bands corresponding to uPA. (c) Effect of hydrostatic pressure on uPA mRNA expression. All results are expressed as mean ± SEM of n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, unpaired Student t test.