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. 2020 Feb 14;10:2634. doi: 10.1038/s41598-020-59462-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of hydrostatic pressure on EMT marker expression by GBM adherent and neurosphere cells. The mRNA expression of EMT markers was quantified using qRT-PCR in (a) U87 and (b) U251 cells after 48 h of hydrostatic pressure as indicated. (c) The mRNA expression of EMT markers was quantified using qRT-PCR in oncosphere cells after 48 h of hydrostatic pressure as indicated. All results are expressed as mean ± SEM of n = 3 independent experiments, *p < 0.05, **p < 0.01, unpaired Student t test.