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. 2020 Jan 23;117(6):3063–3073. doi: 10.1073/pnas.1914308117

Fig. 6.

Fig. 6.

Affinity measurement of TCR 555, 594, and 1005.2.56. Affinity for HLA-DQ2.2:DQ2.2-glut-L1 WT and the P2-Ala, P5-Ala, and P-7Ala epitope mutants were measure via SPR. Two to four independent experiments (with the number of replicates shown as n numbers) were carried out for each of the TCR against each of HLA-DQ2.2-glut-L1 WT and epitope mutants. Binding curve showed represent an independent experiment of TCR 555, TCR 594, TCR 1005.2.56 (labeled as TCR256) binding to HLA-DQ2.2:DQ2.2-glut-L1. All data were combined for each TCR and a one-site specific-binding model was used for curve fitting. HLA-DQ2.5-CLIP was used as negative control and acted as baseline reference value. Error bars, SD. NB, no binding.