Table 1.
DQ2.2-specific TCR sequences
| No. | Donor | TRAV | TRAJ | CDR3a | TRBV | TRBD | TRBJ | CDR3b | No. of cells | TCC |
| 1 | 1005 | 12-3 | 34 | CAMTLNTDKLIF | 4-2 | 1 | 1-2 | CASSLQGGDYGYTF | 1 | |
| 2 | 1005 | 38-1*01 | 32*02 | CAFMKEDGGATNKLIF | 20-1*01 | 1*02 | 2-5*01 | CSASPGNTGVAQYF | 5 | 1005.2.54 and 1005.2.60 |
| 3 | 1005 | 26-1 | 48 | CIVYLSNFGNEKLTF | 5-5 | 2 | 2-2 | CASSLIGGGELFF | 1 | — |
| 4 | 1005 | 29/DV5 | 42 | CAASAHYGGSQGNLIF | 12-5 | 1 | 1-1 | CASGPGTATEAFF | 1 | — |
| 5 | 1005 | 38-2/DV8 | 58 | CAYRSQETSGSRLTF | 20-1 | 1 | 2-5 | CSASWLGFGETQYF | 9 | — |
| 6 | 1005 | 38-2/DV8 | 58 | CAYTPLETSGSRLTF | 20-1 | 1 | 2-7 | CSARAPITGRFYEQYF | 4 | — |
| 7 | 1005 | 21 | 42 | CAVRPGGSQGNLIF | 4-2 | 2 | 2-5 | CASSLTTSEETQYF | 74 | — |
| 29/DV5 | 30 | CAASADLTNRDDKIIF | ||||||||
| 8 | 1005 | 38-1 | 42 | CAFMKGSYGGSQGNLIF | 7-8 | 1 | 2-7 | CASSRTANYEQYF | 28 | — |
| 40 | 40 | FLLGITSGTYKYIF | ||||||||
| 9 | 1005 | 26-1 | 54 | CIVRVAEGAQKLVF | 20-1 | 2-5 | CSATKETQYF | 1 | — | |
| 10 | 1005 | 26-1 | 54 | CIVRVAEGAQKLVF | 20-1 | 1 | 2-5 | CSATKETQYF | 3 | — |
| 11 | 1005 | 21*02 | 31*01 | CAVHTGARLMF | 7-3*01 | 1*01 | 2-3*01 | CASSHGASTDTQYF | NA | 1005.2.56, 1005.2.57, 1005.2.58, 1005.2.62, and 1005.2.65 |
| 12 | 555 | 26-1 | 54 | CIVRVAEGAQKLVF | 20-1 | — | 2-5 | CSATKETQYF | 3 | — |
| 13 | 555 | 6 | 26 | CALVRDYGQNFVF | 7-2 | 2 | 2-7 | CASSTSGGGARSYEQYF | 1 | — |
| 14 | 555 | 1-1*01 | 9*01 | CAWTGGGGFKTIF | 6-2*01 | 2*01 | 2-7*01 | CASRKPGGDYEQYF | 80 | 555A.1.4.38 |
| 15 | 555 | 21 | 58 | CAVIGTSGSRLTF | 7-3 | 1 | 2-3 | CASSSGAATDTQYF | 166 | — |
| 16 | 594 | 26-1 | 54 | CIVRPAQGAQKLVF | 29-1 | 2 | 1-2 | CSVSSGGEGGNGYTF | 1 | — |
| 17 | 594 | 9-2*01 | 57*01 | CASPQGGSEKLVF | 11-2*01 | 1*01 | 1-1*01 | CASSSGGWGGGTEAFF | 62 | 594.5.2.6† |
| 27 | 21 | CAGRDDFNKFYF | ||||||||
| 18 | 627 | 6 | 39 | CALGLNNNAGNMLTF | 10-3 | 1 | 2-5 | CAISPRQAETQYF | 1 | — |
| 19 | 627 | 21 | 11 | CAASGYSTLTF | 11-1 | 1 | 2-3 | CASTHGAGSDTQYF | 108 | — |
| 20 | 627 | 3*01 | 8*01 | CAVSGNTGFQKLVF | 28*01 | 2*02 | 1-5*01 | CASTSRGGNSNQPQHF | NA | 627.1.3.199 |
| DV1*01 | 12*01 | CALGATVMDSSYKLIF |
The table shows the total overview of unique TCRαβ paired sequences generated from high-throughput sequencing of HLA-DQ2.2:DQ2.2-glut-L1 tetramer-sorted single T cells and/or by Sanger sequencing of DQ2.2-glut-L1–specific TCCs. Due to uncertainty in the allele assignment in the TCRαβ sequences generated from single-cell TCR sequencing approach, the alleles for the V, D, and J genes are not shown for the TCRαβ sequences observed only in the single-cell TCR sequencing data. Three TCRαβ sequences generated from single-cell TCR sequencing (nos. 2, 14, and 17) were also found to be expressed by the DQ2.2-glut-L1–specific TCCs, which are mentioned in rightmost column. Two of the TCRαβ sequences (nos. 11 and 20) were observed only by Sanger sequencing of DQ2.2-glut-L1–specific TCCs (last column) and not observed in TCR sequencing data of HLA-DQ2.2:DQ2.2-glut-L1 tetramer sorted cells.
By performing Sanger sequencing, we observed that TCC594.5.2.6 expresses single TCRα chain (TRAV9-2*01/TRAJ57*01). However, by performing single-cell TCR sequencing, we were able to identify T cells that expressed an additional TCRα chain (TRAV27/TRAJ21).