Effects of Fatp4 disruption on the expression levels of keratinocyte differentiation markers and acylceramide synthesis-related genes. Total RNA was prepared from the epidermis of WT (n = 3) and Fatp4 KO (n = 3) mice at postnatal day 0. Real-time quantitative RT-PCR was performed on Hprt1, keratinocyte differentiation markers (Krt14, Krt10, Flg, and Ivl) (A), acylceramide synthesis-related genes (Pnpla1, Cers3, Cyp4f39, and Abhd5) (B), Elovl family members (Elovl1–7) (C), and Fatp family members (Fatp1–6) (D). Values represent the means ± SD and indicate the relative amounts to Hprt1. Statistically significant differences are indicated (two-tailed Student’s t test; *P < 0.05; **P < 0.01).