Fig. 2.
Expression of repressive cldn5-related transcription factor FoxO1 is reduced in the endothelium of resilient mice. (A) Experimental timeline of a 10-d CSDS paradigm, SI test, and tissue collection for RNAscope in situ hybridization. (B) SI ratio of CTRL, SS, and RES mice (one-way ANOVA: F2,15 = 12.46; ***P = 0.0006, n = 6 mice/group). (C) Representative images of FoxO1 expression in the NAc which is reduced in RES mice (two-way ANOVA: phenotype × n clusters interaction effect F12,63 = 4.856; ***P < 0.0001, n = 2,306 to 3,657 cells from four mice/group) (Scale bar, 10 μm) (D). Nonendothelial cells are identified in light gray in the pie charts and endothelial cells, double labeled with CD31, split between shell (white for CTRL, dark gray for SS, pink for RES) and core (white for CTRL, black for SS, red for RES) subregions of the NAc. If one- or two-way ANOVA statistical test was significant, Bonferroni posttests were performed with *P < 0.05; **P < 0.01; ***P < 0.001.